Автор: Свинин Антон Олегович
| Название: | A mollusk Planorbarius corneus is an intermediate host of the infectious agent of rostand’s “anomaly p” in green frogs | 
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| Тематика: | биология | |
| Вид ресурса: | электронная копия бумажного издания | |
| Тип ресурса: | Статья | |
| Целевое назначение: | Научное | |
| Целевая аудитория: | Исследователь | |
| Аннотация: | Here we present the first new data about the mysterious "anomaly P" of green frogs (genus Pelophylax) in about 50 years. We established that the gastropod Planorbarius corneus could be an intermediate host (or vector) of the infectious agent of the anomaly P. Symmetrical cases of polydactyly, the anomaly "cross" and heavy cases of the anomaly P, which were previously found in natural populations in the European part of Russia and recently obtained in laboratory, can be caused by this infectious agent. As the most probable cause, we assume a species of trematodes, for which the first intermediate host is P. corneus, from which they infest tadpoles of green frogs. | |
| Дата издания: | 2019 | |
| Дата загрузки: | 02.12.2020 | |
| Издательство: | Folium Publishing Company | |
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ИР получен из базы данных "Scopus" автоматически
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| Название: | Diversity of trematodes from the amphibian anomaly P hotspot: Role of planorbid snails | 
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| Тематика: | биология | |
| Вид ресурса: | электронная копия бумажного издания | |
| Тип ресурса: | Статья | |
| Целевое назначение: | Научное | |
| Целевая аудитория: | Исследователь | |
| Аннотация: | Trematode infection of the second intermediate hosts can lead to changes in their fitness and, as a result, a change in the invasion rate of animal communities. It is especially pronounced during the invasion of parasite species that reduce activity due to the manipulation of hosts through the changes of their morphology and physiology. One of these cases is an anomaly P syndrome hotspot found in some populations of water frogs and toads in Europe caused by the trematode Strigea robusta metacercariae. The occurrence of pathogen and their participation in ecosystems are intrigues questions in the anomaly P phenomenon, as well as the role of planorbid snails that serve as the first intermediate hosts for many trematode species. Herein, we focused on trematodes spectra from planorbid snails and amphibians from the anomaly P hosts with the aim to undetected interactions between the pathways of parasites. Emerging cercariae of 6802 planorbid snails of dominant species (Planorbarius corneus, Planorbis planorbis, and Anisus spp.) were detected by both morphological and molecular methods in seven waterbodies in Privolzhskaya Lesostep Nature Reserve (Russia). A total of 95 sequences of 18 species were received, and 48 sequences were unique and did not present in any genetic databases. The 18 species of trematodes from snails and 14 species of trematodes from amphibian hosts (Pelophylax ridibundus; Ranidae; Anura) were detected. Three species (Echinostoma nasincovae, Tylodelphys circibuteonis and Australapatemon burti) was new for the trematode fauna of the Middle Volga River region and Russia as a whole. Eleven species of parasitic flatworms have amphibians in their life cycles and nine species used amphibians as metacercariae hosts: Echinostoma nasincovae, E. miyagawai, Echinoparyphium recurvatum, Tylodelphys circibuteonis, Neodiplostomum spathula, Paralepoderma cloacicola, Macrodera longicollis, Strigea robusta, and Strigea strigis. The occurrence of trematode species from planorbid mollusks and frogs were compared. | |
| Дата издания: | 2023 | |
| Дата загрузки: | 23.10.2023 | |
| Издательство: | Public Library of Science | |
| Название: | Effects of minimum Strigea robusta (Digenea: Strigeidae) cercariae doses and localization of cysts on the anomaly P manifestation in Pelophylax lessonae (Anura: Ranidae) tadpoles |
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| Тематика: | биология | |
| Вид ресурса: | электронная копия бумажного издания | |
| Тип ресурса: | Статья | |
| Целевое назначение: | Научное | |
| Целевая аудитория: | Исследователь | |
| Аннотация: | The anomaly P is a mass morphological anomaly found in some populations of anuran amphibians (water frogs of the genus Pelophylax and toads of the genera Bufo and Bufotes) caused by the parasitic flatworm Strigea robusta. Minimum dose of cercariae for the appearance of the anomaly P remains unknown. However, it is important information for understanding of host population dynamics after invasion and the effects of the parasite on the second intermediate hosts. Herein, the invasion properties of S. robusta in Pelophylax lessonae tadpoles (Anura: Ranidae) and minimum dose for appearance of mild and severe forms of the anomaly P syndrome were described after direct experiments with certain numbers of cercariae exposure. Experimental groups of tadpoles have been exposed to eight doses of cercariae (2, 4, 6, 8, 10, 12, 14 and 16). A total of 63.8% tadpoles survived to the end of this experiment. It was revealed that a mild form of the anomaly P (polydactyly) can appear after infection by two cercariae, while the severe form traits appear after infection by four cercariae. The mean number of detected encysted metacercariae was reached to 53.5%. Differences in infection rates can be explained by the presence of an individual immune response in tadpoles or by the presence of different genetic lineages of the parasite infecting the same snail, which have different infectious potential. Low doses of infection leading to the induction of anomalies characterize S. robusta as a highly pathogenic species for amphibian species that are susceptible to infection and show an abnormal phenotype. | |
| Дата издания: | 2023 | |
| Дата загрузки: | 23.10.2023 | |
| Издательство: | Springer Nature Switzerland AG | |
| Название: | Granulomatous inflammations in the intestine of pelophylax ridibundus (Anura: Ranidae) caused by brandesia turgida (plathelminthes: Digenea) | 
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| Тематика: | биология | |
| Вид ресурса: | электронная копия бумажного издания | |
| Тип ресурса: | Статья | |
| Целевое назначение: | Научное | |
| Целевая аудитория: | Исследователь | |
| Аннотация: | The report provides data on granulomatous inflammation in the small intestine of Pelophylax ridibundus, observed in exophytic outgrowths (diverticulums) of duodenum and caused by Brandesia turgida. Amphibians were collected from Protected Areas (Privolzhskaya Lesostep’ State Nature Reserve, Penza region, and Sosnovaya Roscha Forest Park near the town Yoshkar-Ola) during the field seasons of 2011–2018. An histological examination was made according to standard histological procedures; we used hematoxylin and eosin stain. The trematode Brandesia turgida was found not only in the Lieberkühn glands, but also in the serosa. Noncaseating epithelioid granulomas were localised in the serosa. Macrophages and single multinuclear PirogovLanghans giant cells were also detected in the serosa. Granular decay, puckering of nuclei and nuclear detritus were detected. Marked leukocyte infiltration with a significant predominance of eosinophils was observed. The periphery of such granulomas can be characterised by a lymphohistiocytic profile. Mycobacterial infection was not detected. The obtained data describe a new case of granulomatous inflammation in amphibians. | |
| Дата издания: | 2020 | |
| Дата загрузки: | 25.10.2020 | |
| Издательство: | Fund for Support and Development of Protected Areas | |
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ИР получен из базы данных "Scopus" автоматически
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| Название: | Multiplex PCR assay to distinguish among three widespread brown frog species |
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| Тематика: | биология | |
| Вид ресурса: | электронная копия бумажного издания | |
| Тип ресурса: | Статья | |
| Целевое назначение: | Научное | |
| Целевая аудитория: | Исследователь | |
| Аннотация: | Here we present a multiplex PCR assay to distinguish three widespread brown frog species, Rana temporaria, R. arvalis and R. amurensis, based on amplified fragment length. The system consists of four primers to the mitochondrial cytochrome c oxidase subunit I gene, with one universal reverse primer and three species-specific forward primers. Therefore, the PCR gives a fragment of a known specific length, which can be identified by electrophoresis in standard agarose gel. The efficiency of the developed assay was confirmed using both pre-identified and unidentified samples. | |
| Дата издания: | 2024 | |
| Дата загрузки: | 13.10.2024 | |
| Издательство: | Springer Verlag | |
| Название: | New records of the anomaly P syndrome in two water frog species (Pelophylax ridibundus and P. Lessonae) in Russia | 
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| Тематика: | биология | |
| Вид ресурса: | электронная копия бумажного издания | |
| Тип ресурса: | Статья | |
| Целевое назначение: | Научное | |
| Целевая аудитория: | Исследователь | |
| Аннотация: | The "anomaly P" was described in Palearctic water frogs of the genus Pelophylax by Jean Rostand as complex morphological anomalies of water frogs, including polydactyly, brachymely, hind limb oedema, bone outgrowths, spikes, flexions and additional limbs in the inguinal region. In 2016, the anomaly P syndrome was rediscovered in central Russia, confirming the hypothesis concerning its wider distribution. Here, three new records of this syndrome in two species of western Palearctic water frog from Russia are described. | |
| Дата издания: | 2019 | |
| Дата загрузки: | 23.11.2020 | |
| Издательство: | PENSOFT PUBLISHERS | |
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ИР получен из базы данных "Scopus" автоматически
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| Название: | Strigea robusta (Digenea: Strigeidae) infection effects on the gonadal structure and limb malformation in toad early development | 
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| Тематика: | биология | |
| Вид ресурса: | электронная копия бумажного издания | |
| Тип ресурса: | Статья | |
| Целевое назначение: | Научное | |
| Целевая аудитория: | Исследователь | |
| Аннотация: | The anomaly P is a mass morphological anomaly reported in some water frog populations across Europe. It was found that polydactyly is only a mild attenuated form of heavy cases of the anomaly P syndrome, which have strong deformations of the hindlimbs and, partly, forelimbs. It was shown that the anomaly P is caused by the trematode Strigea robusta and this syndrome can be considered as a special case of strigeosis in amphibians. The anomaly P for a long time considered to be specific for water frogs of the genus Pelophylax. Herein, we describe polydactyly and heavy forms of the anomaly P syndrome in toads of the genera Bufo and Bufotes, as a result of exposure to S. robusta cercariae. A total of 150 tadpoles of Bufo bufo, 60 tadpoles of Bufotes viridis, and 60 tadpoles of Bufotes baturae were divided into five experimental and four control groups (30 tadpoles in each group). All anomalies in the toads were similar to those observed in water frogs. The survival of tadpoles in the experimental groups was 76%. The anomaly P was observed in 57.9% of toad tadpoles (51.8% of mild forms and 6.1% of heavy forms). The occurrence of the anomaly P varied among groups from 19% to 78%. Heavy forms of the anomaly P were found in all experimental groups. We described rare asymmetrical cases of the anomaly P. According to severe modification of limb morphology, we supposed changes of gonadal morphology (any modifications of the germ and somatic cells). The gonadal development of infected tadpoles was however the same as in uninfected toad tadpoles, and heterochromatin distribution within gonocytes had no differences as well. It seems like the parasite doesn't have any effect on the gonadal development of the toads. The lack of heavy forms in natural populations of toads, as well as a development of gonads were discussed. | |
| Дата издания: | 2022 | |
| Дата загрузки: | 02.11.2022 | |
| Издательство: | Wiley | |
| Название: | Strigea robusta causes polydactyly and severe forms of Rostand's anomaly P in water frogs | 
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| Тематика: | биология | |
| Вид ресурса: | электронная копия бумажного издания | |
| Тип ресурса: | Статья | |
| Целевое назначение: | Научное | |
| Целевая аудитория: | Исследователь | |
| Аннотация: | Cases of polydactyly in natural populations of amphibians have attracted great interest from biologists. At the end of the 1940s, the French biologist Jean Rostand discovered a polymorphic syndrome in some water frog (Anura: Pelophylax) populations that included polydactyly and some severe morphological anomalies (he called it 'anomaly P'). The cause of this anomaly remains unknown for 70 years. In a previous study, we obtained anomaly P in the laboratory in tadpoles of water frogs that developed together with molluscs Planorbarius corneus (Mollusca: Gastropoda) collected in the field. We thus proposed the 'trematode hypothesis', according to which the infectious agent responsible for anomaly P is a trematode species.MethodsMetacercariae from tadpoles with anomaly P were identified using ITS2 gene sequencing as Strigea robusta (Trematoda: Strigeidae). To verify teratogenic features of the species, cercariae of S. robusta were tested for the possibility to cause anomalies. Identification of cercariae species was made using morphological and molecular methods (sequencing of ITS2 and 28S rRNA). The tadpoles were exposed to parasites at four doses of cercariae (control, low, medium and high) and divided into two groups: "early" (at 25-27 Gosner stages) and "late" (at 29-34 Gosner stages) exposure. Results A total of 58 (72.5%) tadpoles survived until metamorphosis under the dose-dependent experiment with the trematode S. robusta. Differences in the survival rates were observed between the exposed and unexposed tadpoles both in the group of "early" tadpoles and "late" tadpoles. The exposure of tadpoles to the cercariae of S. robusta induced anomaly P in 82% of surviving tadpoles. The severe forms developed only in "early" stages under all doses of cercariae exposure. Polydactyly predominantly developed in the "late" stages; under a light exposure dose, polydactyly also developed in "early" tadpoles. Laboratory-hatched tadpoles reared together with infected snails had different rates of survival and complexity of deformations associated with the period of coexistence. ConclusionsThe experiments with direct cercariae exposure provide compelling evidence that S. robusta leads to anomaly P in tadpoles of water frogs. The manifestation of anomaly P turned out to be dependent on the stage of development, cercariae dose, and the location of the cysts. | |
| Дата издания: | 2020 | |
| Дата загрузки: | 23.11.2020 | |
| Издательство: | BioMed Central Ltd. | |
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ИР получен из базы данных "Scopus" автоматически
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| Название: | The incidence of the anomaly P syndrome in water frogs (Anura, Ranidae, Pelophylax) from the Middle Volga River (Russia) |
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| Тематика: | биология | |
| Вид ресурса: | электронная копия бумажного издания | |
| Тип ресурса: | Статья | |
| Целевое назначение: | Научное | |
| Целевая аудитория: | Исследователь | |
| Аннотация: | The anomaly P is a widespread morphological anomaly, which occurs in some groups of amphibians, caused by the trematode parasite Strigea robusta (Digenea: Strigeidae). This anomaly has been previously recorded in water frogs of the genus Pelophylax and toads of the genera Bufo and Bufotes. The anomaly P includes symmetrical polydactyly cases as a mild attenuated form of the complex syndrome, which in severe cases includes strong deformations of hindlimbs and forelimbs. Strigea robusta has a complex 3-host life cycle using planorbid mollusks as the first intermediate hosts, amphibian larvae as the second intermediate hosts, and anatid birds as the definitive hosts. Herein, we described new records of the anomaly P syndrome in water frogs of the genus Pelophylax from the northeastern parts of their ranges. Symmetrical polydactyly (as a mild form of the anomaly P syndrome) was found in 30 individuals of three species of water frogs from seven localities: in 25 individuals of P. lessonae from four waterbodies, in four individuals of P. ridibundus from three waterbodies, and one individual of P. esculentus. In Gusevo pond, three individuals of P. lessonae with severe cases of the syndrome were found. This is the first record of the anomaly P in reliably identified hybridogenetic edible frogs (P. esculentus) that have been identified in nature. Additionally, we provided new data about the occurrence of the anomaly P and the prevalence of the trematode S. robusta in mollusks taken from two water bodies where anomalous water frogs were found. | |
| Дата издания: | 2023 | |
| Дата загрузки: | 23.10.2023 | |
| Издательство: | PENSOFT PUBLISHERS | |
| Название: | Молекулярно-генетическая характеристика гибридов зеленых лягушек на востоке ареала | 
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| Тематика: | биология | |
| Вид ресурса: | электронная копия бумажного издания | |
| Тип ресурса: | Статья | |
| Целевое назначение: | Научное | |
| Целевая аудитория: | Исследователь | |
| Дата издания: | 2019 | |
| Дата загрузки: | 10.11.2019 | |
| Издательство: | Марийский государственный университет | |
| Название: | Синдром аномалии P у зеленых лягушек: история открытия, морфологические особенности и возможные причины возникновения. | 
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| Тематика: | биология | |
| Вид ресурса: | электронная копия бумажного издания | |
| Тип ресурса: | Статья | |
| Целевое назначение: | Научное | |
| Целевая аудитория: | Исследователь | |
| Аннотация: | Аномалия P у зеленых лягушек была впервые найдена во Франции в 1952 г. французским писателем и ученым Жаном Ростаном. При легкой форме проявления она включает в себя полидактилию, тогда как сложные морфологические преобразования затрагивают передние и задние конечности и включают комплекс признаков: полидактилию, брахимелию, инверсию задних конечностей, небольшие дополнительные конечности, костные выросты, опухоли и отеки в области задних конечностей. Ростан экспериментально показал, что эта аномалия не наследуется и вызывается неким фактором, получаемым из окружающей среды. Она была отмечена только у западно-палерктических представителей зеленых лягушек рода Pelophylax и отсутствовала у других видов амфибий, несмотря на их синтопичное обитание. Тяжелые случаи аномалии P долго не встречались исследователям и были вновь обнаружены спустя полвека с момента их последней регистрации. Новая находка была сделана в 2016 г. в центральной части России, в заповеднике «Приволжская лесостепь». Морфологические особенности аномальных особей лягушек на исследуемой территории оказались схожими с таковыми, описанными Ростаном. Обнаружены симметричная полидактилия, брахимелия, инверсия задних конечностей, отеки бедренной области задних конечностей, небольшие дополнительные конечности в области бедер, костные выросты, а также сопутствующие аномалии – мандибулярная гипоплазия, негнущаяся задняя конечность, несрастание оперкулярной камеры. | |
| Дата издания: | 2020 | |
| Дата загрузки: | 26.11.2020 | |
| Издательство: | Зоологический институт Российской академии наук (Санкт-Петербург) | |
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ИР получен из базы данных "Scopus" автоматически
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| Название: | Трехлетний мониторинг аномалии Ростана в популяциях озерной лягушки «Островцовской лесостепи» | 
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| Тематика: | биология | |
| Вид ресурса: | электронная копия бумажного издания | |
| Тип ресурса: | Статья | |
| Целевое назначение: | Научное | |
| Целевая аудитория: | Исследователь | |
| Дата издания: | 2019 | |
| Дата загрузки: | 10.11.2019 | |
| Издательство: | Марийский государственный университет | |